Trichloracetic Acid

Question: Where can I buy trichloracetic acid? Where can I find trichloracetic acid (TCA) for purchase? What I would want is the same kind doctors would use to treat warts. Im not sure if there are different strengths out there or not. Thank you.

Answer: There are different strengths, all sold here:

Question: How long does Trichloracetic acid take to rid genital warts?(10 pts)? My doctor administered me tricholaracetic acid on a q-tip into me. How long does it take to rid all the warts? Any suggestions?

Answer: hmmm, actually Trichloracetic acid didn't worked for my! it depends of body and immune system too! anyway usually it takes 1-2 weeks ! personally for me worked Wartrol. and with no side effects! You can read more about the product here http://www.wartrol.com/?aid=749165

Question: In extraction of nucleic acid, what is the function of 10% trichloracetic acid? This is during the separation of DNA from RNA and to estimate them separately.

Answer: TCA will precipitate both RNA and DNA longer than 20 nucleotides in length. This can help you precipitate radiolabeled DNA or RNA from primers. TCA will also precipitate protein. See my source for more detail.

Question: Which would you expect to be the stronger acid, trichloracetic acid or trifluoracetic acid?

Answer: trifluoroacetic acid because F is more electronegative than Cl. This would pull electrons from the carboxylic acid group making it more prone to form the negative carboxylic anion and the hydrogen cation. F3C-COOH --> F3C-COO(-) + H(+)

Question: Has any of you out there ever used trichloracetic acid for genital warts??

Answer: This has been used for years. In many cases, one treatment is all you need, but more often, several treatments are needed. It appears that nitrogen works just as well if not better: http://cat.inist.fr/?aModele=afficheN&cp…

Question: to prepare six 2oz bottles of 10% trichloracetic acid, what volume of 36% trichloracetic acid solution is need

Answer: divide the %solution that you want, over %solution that you have and multiply that by the amount you need to make. 10/36 x (6x2) = 3.3 oz

Question: Have u used Trichloracetic acid for genital warts?(SERIOUS PPL ONLY)(10 pts)? I went to the doctor today to get treatment for genital warts and the doctor used a Q-tip and put it inside my vagina where the warts are and around the opening. It burned for a few minutes and went away. However, when I went to the restroom to check down there, that whole area was white. Has anyone every had this happen? What should I do?

Answer: I had the same! its normal and dint worry! :) but actually Trichloracetic acid didn't worked for my! it depends of body and immune system too! personally for me worked Wartrol. and with no side effects! You can read more about the product here http://www.wartrol.com/?aid=749165

Question: whicvh is stronger acid, trichloracetic acid or trifluoracetic acid? Explain brifely. thanx a lot.its urgent!? dont have any other deatil to add.....as i'm pretty bad in chm but urgently need it now..so please ..if u know anythin about it..help me out. greatly appreciated....chaoo

Answer: My guess is that trichloracetic acid is stronger because F has a higher electronegativity, it will pull the electron density from other atoms toward itself, thus it will make the bonds stronger. It's the same reason why HCl is a stronger acid than HF. In fact, HF is not a strong acid because F holds the e- so strongly that it won't allow the H to dissociate. You can refer to CRC handbook for chem and phy to check the pKa values to confirm the final answer.

Question: functions of trichloracetic acid??!!..? Biology Lab!!.. in terms of DNA and RNA

Answer: It is widely used in biochemistry for the precipitation of macromolecules such as proteins, DNA and RNA. Its sodium salt is used as a weedkiller. Solutions containing trichloroacetic acid as an ingredient are used for tattoo removal and the treatment of warts,

Question: Which is the best method to recover a precipitated protein back into its normally folded state? Hi. Can someone help me out here please! Which is the best method to recover a precipitated protein back into its normally folded state? The following solutions were used: -a- 95% Ethanol -b- 25% Ammonium sulphate -c- Solid ammonium sulphate -d- 5% Trichloracetic acid -e-10%Perchloracetic acid -f- 2.5% Perchloracetic acid -g- 10%w/v sodium tungstate in 0.2M H2SO4 -h- Uranyl acetate -i- 0.15M Barium hydroxide and 5% zinc sulphate At first I thought that it could be -g- but am having second thoughts. Could it possibly be -h-? I'm thinking this because uranyl acetate would form a insoluble complex with heavy metals, thus meaning that the structure of the protein would not have changed causing it to denature. The other solutions would cause a structural change in the protein causing it to denature. Am I right in saying this? NB. the protein being spoken about is al Sorry if i haven't written the question is a good enough manner! The thing I'm trying to ask is that, if i wanted to recover a precipitated protein, which of those solutions would i use? Currently I'm thinking barium hydroxide, because it causes aDsorption as opposed to aBsorption. Would I be correct?? Thanks again....and sorry too!

Answer: Don't really understand the question. Is that a list of possible answer choices or are they the different situations that caused the protein to precipitate? Getting a precipitated protein to refold is a multi-step procedure. I'm not familiar with using any of those chemicals. Maybe if I give you my general reasoning process, it will help you figure it out. The first thing I would do is resolubilize the precipitate. I would try a solution with a high concentration of urea first. If that didn't work, I would try guanidine. If that didn't work, I don't know what I would use. Detergents might get the protein solubilized again, but they are a royal pain in the *** to get rid of afterwards, so I would only consider them as a last resort. I might try some sort of organic solvent, but that is starting to get a little crazy. You could add a little heat as well. You might need a reducing agent if there are disulfide bridges present. After the protein is solubilized in its denatured form, the next step is to get it to refold. I would dialyze the solution in multiple steps, headed towards a final buffer that did not have any denaturant in it. The choice of buffer would require a lot of trial and error to get the right pH, ionic strength, metal ions, +/- glycerol, etc. If there are disulfide bridges, you may need a redox active buffer of mixed oxidized and reduced glutathione. Addendum: I don't believe it. Yahoo censors language? I typed three letters there, not three asterixes. Another addendum: Maybe I am still misunderstanding. Is the situation that you *want* to precipitate the protein in order to separate it from everything else? If that is the case, I would go with ammonium sulfate, although I am confused by why it is listed twice. If the solubility of the protein is not already known, then you would titrate in solid ammonium sulfate until the protein salted out. Is albumin the protein you are trying to separate (either you didn't type it or it was cutoff)? If so, 25 % ammonium sulfate is approximately correct (see my source--albumin stays in solution, while other serum globulins salt out). Ammonium sulfate is probably the most commonly used reagent for this purpose. Increasing the ionic strength with a non-chaotropic salt is probably the gentlest approach that you have listed. Alcohols have variable effects on the solubility of different proteins, but I wouldn't use them as general reagents (good for nucleic acids though). TCA will precipitate *most* of your proteins out, so it isn't helpful for separating things. I am not familiar with any of the others. I found one protocol that used perchloroacetic acid for precipitating muscle proteins, but I suspect it might have the same problem as TCA. Sodium tungstate does make proteins precipitate, but I don't know enough about it to say whether it is helpful in a fractional prep. I have no idea how uranyl acetate might help you. As far as I know, it's primary use is for staining for electron microscopy. I think barium hydroxide might be helpful for precipitating out other non-protein stuff and keeping your protein in solution, but using it with a sulfate might cause some problems. Methinks the only precipitate you will get is barium sulfate--but that may not matter if you are trying to keep your protein *in solution*. Again, not very familiar with it.

Question: My genital warts are cured. Is it okay to have sex w/out a condom? i had genital warts & they were cured with trichloracetic acid. They've been gone for about 6 months. Is it okay to have sex w/out a condom and not give it to my partner?

Answer: I always use rubbers... no matter what. It's funny women I have been with never ask me to use one... it's like I care way more then women. I don't get it.

Question: SDS-PAGE tips???? I am currently working in a protein lab. After synthesizing the protein and running it on a FPLC (fast performance liquid chromotography), I have found my protein of interest and now am trying to run it on a gel. Problem is, it is too diluted to see on the gel. I have concentrated it by Trichloracetic acid precipitation, but it still is too dilute. I am currently trying to Silver stain the gel. Any tips on how to concentrate it enough to see in the gel or tips in general??? OK. For clarification, my protein of interest was synthesized with HMBP tag, then cleaved by TEV protease, then purfied by FPLC. For the TCA precipitation, i am using 9:1 concentration of protein mixture to TCA (which is 80%w/v). After letting it sit on ice for 1 hour, I have spinned it down at 4 degrees for 20 minutes at 14,000 rpm (the maximum speed). I am then decanting the supernatant and then mixing in 100 uL of cold 100 % EtOH and letting it sit in ice. Afterwards, i spinned it down and poured out the EtOH. Then I mix in 20 ul of 20mM Tris HCl pH 8.8 and 10 ul of reducing dye. After boiling for 10 minutes, i spin it down and load it into the SDS page. For the silver stain (Pierce SilverSNAP stain), i see some very faint bands now, so hopefully if I increase the amount of protein, i will be able to see any contaminants, etc.

Answer: I guess you do not have an antibody against your protein, do you? Your protein should be quite pure after FPLC... What did you do for dissolving the pellet after TCA precipitation? Did you dissolve it in a small volume? What did you use to dissolve it? I would suggest you to use 8M Urea and shaking for 10-15 min, then boiling. Otherwise, yo ucould use some concentrating columns. If you have an antibody, you could immunoprecipitate it (but I guess you don't, otherwise you would not do FPLC for purification). I'll keep an eye on your question. Could you please post your answers to my questions? Then I can try and help you. Ok. Do you see any residual pellet after boiling? If yes, you could try urea as I mentioned. Is your pellet visible after precipitation? I use acetone for washing instead of EtOH. Maybe you could also try that. If this does not work, I'm afraid you will have to increase the starting material...

Question: HPV[general warts]Ladies only please!!? I just notice that i have small warts a few weeks ago.My doctor only gave me two treatments of trichloracetic acid!? They are going away, they are barely ther.So do you think they will come back?? And can you spread them by wiping yourself or by wearying thong's?? very worried.

Answer: It is really up to your immune system....they can come back but they may never come back. Thongs may cause a bit of irritation...this can give the virus an entry point....wiping might spread the virus if you are having some irritation....possible...maybe??? Treatments only remove the overgrowth of tissue the virus causes. The HPV virus sheds particles with the normal cell shedding our skin goes through.....The virus looks for an entry point; due to the friction of sex - sex is the most common way to spread the virus....but any irritation or scratching could give a point of entry..... I hope that you immune system kicks HPV butt. How often can episodes occur? Some people only have one episode, while others have recurrences When warts are present, the virus is considered active When warts are gone, the virus is latent (sleeping) in the skin cells - it may or may not be contagious at this time Genital warts may or may not return after the first episode A healthy immune system is usually able to clear the virus, or suppress it, over time. Warts may appear within several weeks after sex with someone who has the wart-types of HPV, or it may take several months or years to appear. Or, warts may never appear. This makes it hard to know exactly when or from whom someone got the virus. http://www.ashastd.org/learn/learn_hpv_w…

Question: How Do I Get Over Being Raped and Then Disfigured by the Planned Parenthood Nurse? I was raped in October 07, and contracted genital warts from it. I went to my dr. and I was prescribed an ointment, except the ointment cost $1,200.00. I'm in college and have no income so I couldn't afford it. I decided to go to Planned Parenthood for treatment that cost $40.00. The woman there said I should have a "TCA treatment" and showed me a consent form which stated the only risks were mild redness and itching. Since there were no permanent risks, I decided to try it. However, the nurse botched the treatment and instead of putting the acid (I later found out that TCA stands for the powerful acid trichloracetic acid and was in a very high concentration --85%) only on the warts, she put it on my entire external genitalia. I only had a few warts so as you can imagine, the acid that was applied to all my delicate genital areas severely disfigured me. I had reconstructive surgery for 5,000 and it removed a lot of the burned skin. However, I want to still have some laser surgery to fix the color of the skin, (it's silver in color) but I know its expensive I'm so depressed about this...I actually found a great attorney that's going to get me compensations for my pain and suffering but I just feel like I will always be depressed about this. I want to find a great husband one day and get married how how can I ever be comfortable with sex??? Knowing that I am disgusting down there????? I feel so gross. Everytime I hear people talk about sex I get so depressed because I realize that I don't know if I will ever enjoy sex with my future husband, and that will probably break up our marriage becaues I cant be intimate. How do I get over this???? Please help.

Answer: Well this is something not very many women have actually expierenced, so I can't say I know what you're going through in any manor, but you have to learn how to accept yourself for how you are. Even if you think it is hideous down there, someone who loves you is going to understand and make you feel comfortable about sex. If he doesn't he isn't worth your time. It is hard finding beauty in ourselves, especially if it is so repulsive to us, but the moment you learn to accpet yourself for how you are is the moment you allow everyone else to see how beautiful you really are. I'm sorry this happened to you, I'd go after the bastard that raped you for all his money to repair the damages.

Question: Christians: How Will I Be Able to Have Sex With my Future Husband After This Rape? Please help? I was raped in October 07, and contracted genital warts from it. I went to my dr. and I was prescribed an ointment, except the ointment cost $1,200.00. I'm in college and have no income so I couldn't afford it. I decided to go to Planned Parenthood for treatment that cost $40.00. The woman there said I should have a "TCA treatment" and showed me a consent form which stated the only risks were mild redness and itching. Since there were no permanent risks, I decided to try it. However, the nurse botched the treatment and instead of putting the acid (I later found out that TCA stands for the powerful acid trichloracetic acid and was in a very high concentration --85%) only on the warts, she put it on my entire external genitalia. I only had a few warts so as you can imagine, the acid that was applied to all my delicate genital areas severely disfigured me. I had reconstructive surgery for 5,000 and it removed a lot of the burned skin. However, I want to still have some laser surgery to fix the color of the skin, (it's silver in color) but I know its expensive I'm so depressed about this...I actually found a great attorney that's going to get me compensations for my pain and suffering but I just feel like I will always be depressed about this. I want to find a great husband one day and get married how how can I ever be comfortable with sex??? Knowing that I am disgusting down there????? I feel so gross. Everytime I hear people talk about sex I get so depressed because I realize that I don't know if I will ever enjoy sex with my future husband, and that will probably break up our marriage becaues I cant be intimate. How do I get over this???? Please help. Oct 07 as in October of the year 2007.

Answer: I am very sorry for your difficulty. 2 ways to deal with it: 1] Find spirituality -- sex is not the be all and end all without love so when people brag about sex they are misled. Also, not everyone is having a lot of sex -- though everyone pretends they are. 2] Know that when you find someone who really loves you and deserves you, he won't care about it. When you explain it to him, you will be able to tell by his reaction if he is the one or not. 3] Know that many people have gone through worse. At least it works and you can urinate. At least it was not your face. Or your brain or heart. Good Luck and best wishes to ya :) Edit: I think she meant October, 2007. Hey Nerdy .. long time no see :)

Question: How Do I Get Over This: Rape and as a Result Disfigured Genitals? I was raped in October 07, and contracted genital warts from it. I went to my dr. and I was prescribed an ointment, except the ointment cost $1,200.00. I'm in college and have no income so I couldn't afford it. I decided to go to Planned Parenthood for treatment that cost $40.00. The woman there said I should have a "TCA treatment" and showed me a consent form which stated the only risks were mild redness and itching. Since there were no permanent risks, I decided to try it. However, the nurse botched the treatment and instead of putting the acid (I later found out that TCA stands for the powerful acid trichloracetic acid and was in a very high concentration --85%) only on the warts, she put it on my entire external genitalia. I only had a few warts so as you can imagine, the acid that was applied to all my delicate genital areas severely disfigured me. I had reconstructive surgery for 5,000 and it removed a lot of the burned skin. However, I want to still have some laser surgery to fix the color of the skin, (it's silver in color) but I know its expensive I'm so depressed about this...I actually found a great attorney that's going to get me compensations for my pain and suffering but I just feel like I will always be depressed about this. I want to find a great husband one day and get married how how can I ever be comfortable with sex??? Knowing that I am disgusting down there????? I feel so gross. Everytime I hear people talk about sex I get so depressed because I realize that I don't know if I will ever enjoy sex with my future husband, and that will probably break up our marriage becaues I cant be intimate. How do I get over this???? Please help.

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Question: Which is the best method to recover a precipitated protein back into its normally folded state? Hi. Can someone help me out here please! Which is the best method to recover a precipitated protein back into its normally folded state? The following solutions were used: -a- 95% Ethanol -b- 25% Ammonium sulphate -c- Solid ammonium sulphate -d- 5% Trichloracetic acid -e-10%Perchloracetic acid -f- 2.5% Perchloracetic acid -g- 10%w/v sodium tungstate in 0.2M H2SO4 -h- Uranyl acetate -i- 0.15M Barium hydroxide and 5% zinc sulphate At first I thought that it could be -g- but am having second thoughts. Could it possibly be -h-? I'm thinking this because uranyl acetate would form a insoluble complex with heavy metals, thus meaning that the structure of the protein would not have changed causing it to denature. The other solutions would cause a structurak change in the protein causing it to denature. Am I right in saying this? NB. the protein being spoken about is albumin. Thanks!! I am not making anything. I did a experiment where basically we precipitated proteins in different solutions and now we gotta work out which would be best if we wanted to recover the protein again. So... which solution should i use? ok. Wouldn't ethanol be the best method for PRECIPITATING the Protein so that they are no longer in the solution, as ethanol associates with water more strongly compared to proteins. I would like to know the opposite... which would be best if I wanted recover the protein, so that it is back in the solution. For example, bring back up the protein concentration. Thanks... sorry if i'm being a nuisance!

Answer: A rapid and quantitative isolation of human serum albumin is give here. The present technic is a modification of the Schwert method. In the present method 4 ml. of serum is precipitated with trichloroacetic acid in a final concentration of 5% (w/v). The precipitate is washed with TCA, and after aqueous suspension, ethanol is added to a final concentration of 80%. The undissolved globulin is washed with ethanol and the solubilized albumin used for quantification, characterization, and isotope counting. Cellulose acetate electrophoresis will show a single band, and agar diffusion will produce one precipitate line. you should retreive with 98% efficiency. ------------------------------------ use trichloric acid and ethanol.

Question: Does glycolic acid peel or trichloracetic acid work for tattoo removal?

Answer: Nope, you have to get them removed by laser.