Bromelain

Question: How to prove papain is better than bromelain and actinidin? My project is trying to prove that papain is better than bromelain and actinidin in terms of protein-digesting. We did an experiment on it, but we need research evidence to support it and so far it has been rather difficult. I'll appreciate some help. Thanks.

Answer: Old style photographic film (from the days before digital) had silver grains suspended in a thin film of gelatin on a plastic backing. Strips of this film, put into protease enzyme would clear as the gelatin was digested and the silver particles fell off. The speed of this clearing gave an indication of protease activity Try your three protein-digesters under uniform conditions and time to clear. Result. p.s. you don't set out to PROVE that one enzyme is better, you set out to TEST if one is better. Subtle but important difference. John H

Question: How do you get rid of the enzyme bromelain in the pinapple? I love fresh pineapples but hate it when you eat too many and it cuts your tongue because of the bromelain in it. My friends say to soak it in salt to get rid of it but that makes them taste bad. Anyone know a better way?

Answer: The only way that I know of is heat. I believe heat deactivates/destroys the bromelain, especially about 119 F. Also, cold temperatures (below 40F) reduces the effectiveness of bromelain, but does not destroy the enzyme. So eat the pineapple cold, below 40F, or heat the pineapple to 120F and cool before eating. PS... Fresh pineapple cutting your tongue? You are peeling the pineapple first before eating, arent' you?

Question: How long does it take to see the results of using Bromelain to treat swelling? I've been taking Bromelian in pill form 3 times a day at 500 mg for 2 days. I had surgery 2 weeks ago and i was told that bromelain would help with the swelling, but i see no results =(

Answer: this may be the reason. Bromelain is used in the stomach as an enzyme to digest protein, it doesn't get used as an anti-inflammatory. Life Exensin Foundation...LEF.org ...sells a specially coated Bromelain that does not break down in the stomach, it travels to the intestines where it is absorbed and used as an anti-inflammatory.Call them at 1-800-544-4440...in Florida. Fish oil is also a very potent anti-inflammatory, ask LEF about that also.

Question: Can taking 3,000 mg bromelain daily cause skipped heartbeats? I have been taking about 3,000 mg of bromelain for a long time. Can this cause irregular heartbeat, skipped beats and sometimes at night fast heartbeats?

Answer: Bromelain is one of a group of peptide hydrolases, all thiol proteinases, obtained from pineapple stems and fruit; used in tenderizing meats and in producing hydrolysates of proteins; as a complementary and alternative medicine, it is orally administered in the treatment of inflammation and edema of soft tissues associated with traumatic injury. The daily recommended intake of bromelain is only 500-1500 mg but You are taking way too much for a long time. Overall, bromelain supplementation up to 500 mg has been shown to have no effect on human heart rate or blood pressure; however, increasing doses up to 1500 mg and over have been shown to increase the heart rate proportionately. Some of Its other side effects include nausea, vomiting, diarrhea, menorrhagia (excessively heavy menstrual flow) and possible allergic reactions. I will suggest that You discontinue taking bromelain and visit a medical specialist for heart related check-ups. For more information either call bromelain manufacturers @: 1-800-969-7228 or visit their website @ http://www.bromelain.net Good Health To You !

Question: Will Bromelain act as a preservative after doing it as an ointment? Will Bromelain act as a preservative after making it as an ointment?

Answer: Bromelain is more commonly not used as a preservative... it's a digestive enzyme. More commonly, Potassium Sorbate, Sodium Benzoate, Vitamins C and/or Vitamin E are used as natural preservatives, but creams and ointments sometimes have to use more synthetic preservatives depending on the formulation. I hope I helped!

Question: What does the enzyme bromelain do for pineapples? I am aware of its uses in medicine and such, but what does the enzyme do for the actual pineaple?

Answer: It is not clear, but bromelain is downregulated during ripening.

Question: Which part of the pineapple contains the most bromelain? If it is in the pineapple juice, how can I get the pineapple juice from the fruit?

Answer: Oh, now I get it, its a weight loss thing. The syrup/juice contains the bromeline. Squeezing, pulping, or blending and filtering the pineapple innards should do the trick. It really might just be easier to buy pineapple juice at this point, or eat the pineapple.

Question: How many grams of Bromelain does an average sized pineapple contain? Provide the source of your answer/data.

Answer: Using Wikipedia I found that 100g of pineapple contains approximately 226mg of bromelain enzymes. therefore estimated ratio of bromelain enzymes to pineapple = 1:500 Hope that helps

Question: What is produced when bromelain breaks down collagen? What are the specific products of the reaction?

Answer: In the 1890s, researchers began isolating and studying bromelain, a natural mixture of two proteases (protein-digesting enzymes) found in pineapples. They discovered that bromelain is quite effective at dividing proteins such as the collagen in steak, as well as in your tongue. Although bromelain is found in every part of the pineapple, it's most plentiful in the stem. Most commercially grown pineapple is sliced, canned or juiced. The remains are rich with this corrosive substance, which is then extracted. Powdered bromelain is used for tenderizing meat, as well as treating inflammation, swelling, indigestion and even excessive blood clotting. Bromelain works in these capacities due to its ability to separate amino acids. Amino acids are organic compounds within living cells. Amino acids join by forming peptide bonds, a link that connects one amino acid's amino group with the carboxyl group of another amino acid. When amino acids join through peptide bonds, they form proteins. These proteins then carry out numerous functions in the structure and operation of cells, tissues and organs. In Pursuit of Cold, Clear Beer In addition to tenderizing meat, bromelain is used to "chill-proof" beer. When beer is cooled to about 32 degrees Fahrenheit (zero degrees Celsius), proteins form bonds with other elements in the beer. This doesn't affect the taste of the beer, but it makes it appear cloudy. Bromelain is added to beer to prevent this from happening. Even without chill-proofing, the cloudiness goes away once the temperature of the beer rises. In a store's beer cooler though, consumers are generally going to reach for a clear beer over something that resembles a thick gruel. The compound separates the all-important peptide bonds that link the proteins in collagen. Since the collagen gives the muscle tissue its shape, once it's broken down, the muscle tissue begins losing firmness. If you left meat for a day or two covered with bromelain, it would be noticeably mushy, so much so that you wouldn't want to eat it. When used shortly before cooking, however, bromelain efficiently softens the steak for chewing, but leaves it firm enough to enjoy its taste. The enzymes are neutralized by heat of about 158 degrees Fahrenheit (70 degrees Celsius), so they stop working once cooked.

Question: Where can I get bromelain and barley grass? Hi, Just wondering where I can buy bromelaine and barley grass in Vic, Australia? Do brands like Nature's Own and Herron make them? Any help would be great.

Answer: You can get them from health stores. The supermarkets and chemists and pharmacies that sell vitamins and other dietary supplements don't seem to have bromelain and barley grass.

Question: How could the canning process change bromelain in a pineapple?

Answer: Bromelein is an enzyme. Enzymes are proteins. Proteins are denatured by heat. Canning involves heat.

Question: what is the maximum time you can tenderize tri tip with mccormick meat marinade seasoning mix with bromelain? i heard if you tenderize meat too long it will get mushy. can you tenderize overnight or is that too long. the last couple of times i cokked tri tip on the outdoor bbq it was real tough and i cut the roast before i bbq it.

Answer: You can tenderize meat as long as you want to.

Question: Where i can purchase bromelain tablets in patna bihar? bromelain tablets are oral supplement for bromelain found in pineapple. it is a fat reducing and anti inflammatory element. i read about it in a magazine but the company has no retailer in patna.

Answer: hi chk with http://www.indiadatingzone.cm

Question: What are the different identification tests and storage conditions for bromelain? Can anyone please give me the specific storage temparture for bromelain?

Answer: Are you talking about the element bromine to be stored as a liquid in a glass bottle and in a fridge? Best identification is: weigh it and roughly calculate its density. Must come out around 3 kg/liter and doesn't exist another such heavy liquid, which is at the same time giving out reddish-brown vapors at room temperature that easily. But take care when handling it: is poisonous and very corrosive; can easily damage your nose when smelling it!!! Or is it an organic extract from bromelia plants, what you want to store and characterize? But for such substances I don't have any experience..

Question: is mccormicks meat marinade seasoning mix good for tenderizing tough meat. it says it has bromelain in it? is there any good meat tenderizers out there? also is pounding the meat a good way of tenderizing meat?

Answer: TEA!!! the tanin in tea is a natural tenderizer and not very expensive. It doesn't change the flavor of the meats at all. It does give the gravy on soups, stews, and roasts a delightful brown color. Use left over unsweetened tea on steaks and tougher cuts of beef to marinade in fridge for about 4 hours or more. put large family size teabag in soup pot or stew pot to simmer with the meat. put large family size teabag in roaster with a bit of water to bake in oven. Turn roast about 4 times during cooking and replace water or liquid while cooking. I turn once an hour! Pounding the meat breaks down the texture of the muscles. It does work for tenderizing some things. Another tenderizer is beer!! It does change the flavor of the meat! You can marinade or simmer in beer. Be aware that beer does foam!

Question: Does canned pineapple juice contain enough bromelain to be effective? ? I've heard that the canning process destroys the bromelain enzyme. I've also heard that most is destroyed but not all. Which is true? Does drinking canned pineapple juice have any of the same benefits as fresh pineapple?

Answer: It will have some of it... but not as much as fresh pineapple. When foods are canned commercial, there is commonly some heat used to sterilize the can and contents to make sure that there won't be any growing microbes in the food. That heat also destroys some of the enzymes (including some of the Bromelain), so it won't have as much of an impact for digestion. I'm actually allergic to pineapple (but I love it, so it really sucks). I have a much bigger and faster reaction to fresh pineapple than canned, so I know from personal experience that there is a lot that's lost in canning. However, when I need the benefits from the Bromelain, I actually take it in a supplemental capsule to make sure I get the most without the reaction to the pineapple. Good luck!

Question: Does orange juice contain any bromelain? It's for a biology assignment. We did an experiment but I failed to record some of the results.

Answer: Yes, bromelain is believed to help with the digestion of protein.

Question: will bromelain destory healthy living tissue? I heard bromelain is very good for taking down scar tissue, and plaque and stuff. But is it safe for living tissue, will it also break down healthy living tissue? I brought me some serrapeptase today because according to many various websites they conclude that it only breaks down fiberious tissue, plaque and scar tissue ect. without harming living and healthy tissue. But what about bromelain?

Answer: Bromelain is an enzyme found in Pineapple that has a few different health benefits. When it is taken away from food it acts as an anti-inflammatory. When taken with food it acts as a digestive aid. I have not heard anything about harmful effects on healthy tissue. Hope that helps.

Question: What type of substrate do papain and bromelain react with?

Answer: PROTEINS ARE THE SUBSTRATE AND AMINO ACIDS ARE THE PRODUCT BOTH OF THESE ENZYMES DIGEST PROTEINS Bromelain - Bromelain is an enzyme found in pineapples that breaks down other proteins, such as collagen and muscle fiber. Bromelain is a mixture of sulfur-containing protein-digesting enzymes, called proteolytic enzymes or proteases. Bromelain is mainly comprised of cysteine proteases, with smaller amounts of acid phosphatase, peroxidase, amylase and cellulase. . Papain - Papain is in the dried latex obtained from the papaya fruit (Carica papaya L). Papain hydrolyzes proteins to form oligopeptides and amino acids. Papain has milk-clotting (rennet) and protein digesting properties. Papain has the ability to digest dead tissue without affecting the surrounding live tissue.

Question: Has anyone tried Quercetin & Bromelain for allergy problems? Does it work? How much do you take? Any side effects? Do you take it all through the allergy season or just when bothered by allergies?

Answer: I have.They do help.Also vit. C.I have found avoiding allergenic foods helps with airbourn allergys.